Characterization of Mycobacterium abscessus colony-biofilms based on bi-dimensional images.
John Jairo Aguilera-CorreaYves-Marie BoudehenLaurent KremerPublished in: Antimicrobial agents and chemotherapy (2023)
Mycobacterium abscessus biofilm aggregates have been shown in the lungs of cystic fibrosis patients and are often tolerant to drugs. Herein, we analyzed bi-dimensional images of either fluorescent or Congo red-stained M. abscessus colony-biofilms grown on a membrane to monitor growth and shape of M. abscessus smooth and rough variants. These colony-biofilms responded differently to rifabutin and bedaquiline, thus highlighting the importance of the morphotype to properly address antibiotic treatment in patients with biofilm-related infections.
Keyphrases
- candida albicans
- pseudomonas aeruginosa
- cystic fibrosis
- deep learning
- end stage renal disease
- staphylococcus aureus
- mycobacterium tuberculosis
- biofilm formation
- ejection fraction
- convolutional neural network
- newly diagnosed
- drug resistant
- chronic kidney disease
- optical coherence tomography
- multidrug resistant
- escherichia coli
- copy number
- lung function
- chronic obstructive pulmonary disease
- machine learning
- dna methylation
- patient reported outcomes
- air pollution
- smoking cessation
- fluorescent probe