Excess Ascorbate is a Chemical Stress Agent against Proteins and Cells.
Maria LeheneEva Fischer-FodorFlorina ScurtuNiculina D HădadeEmese GalAugustin Catalin MoțAlina MateiRadu Silaghi DumitrescuPublished in: Pharmaceuticals (Basel, Switzerland) (2020)
Excess ascorbate (as expected in intravenous treatment proposed for COVID-19 management, for example) oxidizes and/or degrades hemoglobin and albumin, as evidenced by UV-vis spectroscopy, gel electrophoresis, and mass spectrometry. It also degrades hemoglobin in intact blood or in isolated erythrocytes. The survival rates and metabolic activities of several leukocyte subsets implicated in the antiviral cellular immune response are also affected. Excess ascorbate is thus an unselective biological stress agent.
Keyphrases
- immune response
- mass spectrometry
- induced apoptosis
- coronavirus disease
- high resolution
- peripheral blood
- sars cov
- cell cycle arrest
- liquid chromatography
- stress induced
- red blood cell
- high dose
- single molecule
- dendritic cells
- signaling pathway
- toll like receptor
- oxidative stress
- gas chromatography
- high performance liquid chromatography
- cell death
- combination therapy
- replacement therapy
- heat stress
- inflammatory response