Nuclear localization of non-structural protein 3 (NS3) during dengue virus infection.
Selvin Noé Palacios-RápaloLuis Adrián De Jesús-GonzálezJosé Manuel Reyes-RuizJuan Fidel Osuna-RamosCarlos Noe Farfan-MoralesAna Lorena Gutiérrez-EscolanoRosa María Del ÁngelPublished in: Archives of virology (2021)
Although dengue virus (DENV) replication occurs in the cytoplasm, the nucleus plays an essential role during infection. Both the capsid protein (C) and non-structural protein 5 (NS5) are translocated into the infected cell nucleus to favor viral replication. Previously, our group reported the nuclear localization of the NS3 protein during DENV infection of mosquito cells; however, the nuclear localization of the DENV NS3 protein in human host cells has not been described. Here, we demonstrated that NS3 is present in the nucleus of Huh7 cells at early infection times, and later, it is mainly located in the cytoplasm.