A Transferrin Triggered Pathway for Highly Targeted Delivery of Graphene-Based Nanodrugs to Treat Choroidal Melanoma.
Bingxin ZhaoKalun DongMimi LinGongxian DongSuyan ShanTom LawsonLu YanWenjing ZhangBingyang ShiShulei ChouMark S BakerYong LiuPublished in: Advanced healthcare materials (2018)
The synthesis of transferrin (Tf)-modified pegylated graphene (PG) and its application as a highly efficient drug delivery carrier for therapy of Ocular Choroidal Melanoma-1 (OCM-1) cells is presented. For the first reported time, nanoscaled PG is prepared using an environmentally friendly ball-milling technique. The unique 2D nanostructure obtained using this PG synthesis approach offers considerable advantages in terms of drug loading and delivery, as well as the conjugation of Tf to PG providing a more targeted delivery vehicle. A highly efficient targeted pathway toward OCM-1 cells triggered by an affinity between Tf and Tf receptors expressed on the surface of OCM-1 cells is reported first here. PG-Tf is observed to easily anchor anticancer drugs such as doxorubicin via π-π stacking. This work performs a Transwell two cells coculture experiment, a 3D in vitro tumor model, and an in vivo mouse model with OCM-1 tumors to demonstrate the composite's therapeutic superiority over conventional systems for the targeted delivery and controlled release of antitumor drugs.
Keyphrases
- highly efficient
- induced apoptosis
- cell cycle arrest
- drug delivery
- mouse model
- cancer therapy
- emergency department
- oxidative stress
- optical coherence tomography
- stem cells
- mass spectrometry
- signaling pathway
- mesenchymal stem cells
- cell therapy
- electronic health record
- room temperature
- walled carbon nanotubes
- age related macular degeneration
- basal cell carcinoma