Enhanced ethanol formation by Clostridium thermocellum via pyruvate decarboxylase.
Liang TianSkyler J PerotShuen HonJilai ZhouXiaoyu LiangJason T BouvierAdam M GussDaniel G OlsonLee R LyndPublished in: Microbial cell factories (2017)
The best PDC enzyme was from Acetobactor pasteurianus. A strain of C. thermocellum expressing the pdc gene from A. pasteurianus and the adhA gene from T. saccharolyticum was able to produce 21.3 g/L ethanol from 60 g/L cellulose, which is 70% of the theoretical maximum yield.