Glyphosate resistance in Ambrosia trifida: Part 2. Rapid response physiology and non-target-site resistance.
Marcelo L MorettiChristopher R Van HornRenae RobertsonKabelo SegobyeStephen C WellerBryan G YoungWilliam G JohnsonR Douglas SammonsDafu WangXia GeAndré d' AvignonTodd Adam GainesPhilip WestraAmanda C GreenTaylor JefferyMackenzie A LespéranceFrançois J TardifPeter H SikkemaJ Christopher HallMichael D McLeanMark B LawtonBurkhard SchulzPublished in: Pest management science (2017)
The GR RR mechanism of resistance is not associated with vacuole sequestration of glyphosate, and the observed reduced translocation is likely a consequence of rapid tissue death. Rapid cell death was inhibited by exogenous application of aromatic amino acids phenylalanine and tyrosine. The mechanism by which these amino acids inhibit rapid cell death in the GR RR phenotype remains unknown, and it could involve glyphosate phytotoxicity or other agents generating reactive oxygen species. Implications of these findings are discussed. The GR RR mechanism is distinct from the currently described glyphosate TSR or NTSR mechanisms in other species. © 2017 Society of Chemical Industry.