Rapid detection of the New Delhi metallo-b-lactamase 1 (NDM-1) gene by loop-mediated isothermal amplification (LAMP).
Mirna Giselle MoreiraLidia Miranda BarretoVera Lúcia Dos SantosAndrea Souza MonteiroVandack NobreSimone Gonçalves Dos SantosPublished in: Journal of clinical laboratory analysis (2017)
The lamp technique seems to be an excellent option for the rapid detection of blaNDM-1. The amplification time is much shorter than other molecular techniques, the PCR machine is not necessary, it is easy of implementation and costs is low.
Keyphrases
- loop mediated isothermal amplification
- klebsiella pneumoniae
- multidrug resistant
- escherichia coli
- gram negative
- sensitive detection
- primary care
- healthcare
- nucleic acid
- copy number
- deep learning
- genome wide
- quality improvement
- machine learning
- quantum dots
- single molecule
- transcription factor
- gene expression
- label free
- dna methylation