Cross-Link-Functionalized Nanoparticles for Rapid Excretion in Nanotheranostic Applications.
Zhuoran MaFeifei WangYeteng ZhongFelix SalazarJiachen LiMingxi ZhangFuqiang RenAnna M WuHongjie DaiPublished in: Angewandte Chemie (International ed. in English) (2020)
Most NIR-IIb fluorophores are nanoparticle-based probes with long retention (≈1 month or longer) in the body. Here, we applied a novel cross-linked coating to functionalize core/shell lead sulfide/cadmium sulfide quantum dots (PbS/CdS QDs) emitting at ≈1600 nm. The coating was comprised of an amphiphilic polymer followed by three crosslinked amphiphilic polymeric layers (P3 coating), imparting high biocompatibility and >90 % excretion of QDs within 2 weeks of intravenous administration. The P3 -QDs were conjugated to an engineered anti-CD8 diabody (Cys-diabody) for in vivo molecular imaging of CD8+ cytotoxic T lymphocytes (CTLs) in response to anti-PD-L1 therapy. Two-plex molecular imaging in combination with down-conversion Er nanoparticles (ErNPs) was performed for real-time in vivo monitoring of PD-L1 positive tumor cells and CTLs with cellular resolution by non-invasive NIR-IIb light sheet microscopy. Imaging of angiogenesis in the tumor microenvironment and of lymph nodes deep in the body with a signal-to-background ratio of up to ≈170 was also achieved using P3 -QDs.
Keyphrases
- quantum dots
- photodynamic therapy
- fluorescence imaging
- single molecule
- lymph node
- drug release
- high resolution
- sensitive detection
- fluorescent probe
- living cells
- drug delivery
- energy transfer
- small molecule
- nk cells
- endothelial cells
- heavy metals
- high dose
- gestational age
- vascular endothelial growth factor
- loop mediated isothermal amplification
- breast cancer cells
- sentinel lymph node
- risk assessment
- bone marrow
- locally advanced
- single cell
- tandem mass spectrometry
- rectal cancer