Identification and validation of specific B-cell epitopes of hantaviruses associated to hemorrhagic fever and renal syndrome.
Fernando de Paiva ConteBianca Corrêa TinocoThiago Santos ChavesRenata Carvalho de OliveiraJanaina Figueira MansurRonaldo Mohana-BorgesElba Regina Sampaio de LemosPatricia Cristina da Costa NevesRodrigo Nunes Rodrigues-da-SilvaPublished in: PLoS neglected tropical diseases (2019)
In silico analysis revealed nine potential immunogenic linear B-cell epitopes from SHNP; among them, SHNP(G72-D110) and SHNP(P251-D264) showed a high degree of sequence conservation among HFRS-related orthohantavirus and were experimentally validated against rSHNP-IMS and negatively validated against rJHNP-IMS. Taken together, we identified and validated two potential antigenic B-cell epitopes on SHNP, which were conserved among HFRS-associated orthohantavirus and could be applied to the development of novel immunodiagnostic tools for orthohantavirus surveillance.