Third-Generation Single-Molecule Sequencing for Preimplantation Genetic Testing of Aneuploidy and Segmental Imbalances.

Vivienne J Tan Timing LiuZainul ArifinBeatrice PakArnold S C TanSimin WongChiea Chuen Khor Henry Yang Caroline G L Lee Zhongwei Huang Mahesh Arjandas Choolani Samuel S Chong

Published in: Clinical chemistry (2023)

Up to 24 SurePlex-amplified single-cell, multicell, or trophectoderm samples could be sequenced in a single MinION flow-cell for subsequent preimplantation genetic testing for aneuploidy or structural rearrangements (PGT-A/SR) analysis, with results obtainable in ≤3 days and at per-sample costs that are competitive with commercial offerings. Nanopore's third-generation single-molecule sequencing represents a viable alternative to current commercial NGS-based PGT-A solutions for aneuploidy and segmental imbalance (≥10 Mb) screening of single-/multicell or trophectoderm biopsy samples.

Keyphrases

single molecule
single cell
rna seq
high throughput
living cells
atomic force microscopy
bone marrow
stem cells
high resolution
fine needle aspiration
cell therapy