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Aneuploidy screening of embryonic stem cell clones by metaphase karyotyping and droplet digital polymerase chain reaction.

Gemma F CodnerLoic LindnerAdam CaulderMarie Wattenhofer-DonzéAdam RadageAnnelyse MertzBenjamin EisenmannJoffrey MiannéEdward P EvansColin V BeecheyMartin D FrayMarie-Christine BirlingYann HéraultGuillaume PavlovicLydia Teboul
Published in: BMC cell biology (2016)
We validated a PCR-based method as an alternative to classical karyotype analysis. This technique enables laboratories that are non-specialist, or work with large numbers of clones, to precisely screen ES cells for the most common aneuploidies prior to microinjection to ensure the highest level of germline transmission potential. The application of this method allows early exclusion of aneuploid ES cell clones in the ES cell to mouse conversion process, thus improving the chances of obtaining germline transmission and reducing the number of animals used in failed microinjection attempts. This method can be applied to any other experiments that require accurate analysis of the genome for copy number variation (CNV).
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