Effects of Tomato Juice Intake on Salivary 8-Oxo-dG Levels as Oxidative Stress Biomarker after Extensive Physical Exercise.
Ali Pour KhavariSiamak HaghdoostPublished in: Oxidative medicine and cellular longevity (2020)
Reactive oxygen species (ROS) at a normal level are important molecules involved in several cellular processes including immune response and cell signalling. Overproduction of ROS may lead to elevated oxidative stress and consequently to age-related diseases. Most of the studies related to oxidative stress in humans have been done on blood samples. However, blood sampling might be painful, requires special qualified personnel, and has to be performed at medical centers. An alternative to blood is saliva. Saliva sampling is noninvasive and can be performed by the donor. Biomarker determination in saliva is becoming an important part of laboratory diagnosis, but method development is needed before it can be used in the clinics. In the present investigation, 16 donors performed extensive physical exercise by cycling and keeping their heart rate at 80% of maximum for 20 minutes. The physical activity was repeated 3 times: before tomato juice intake, after daily intake of 100 ml tomato juice during 3 weeks, and finally 3 weeks after finishing tomato juice intake (washout period). The level of the stress biomarker, salivary 8-oxo-dG, was determined before and after the physical activity. The results indicate that (a) 20 min extensive physical activity increases the level of 8-oxo-dG in saliva significantly (p = 0.0078) and (b) daily intake of 100 ml tomato juice may inhibit (p = 0.052) overproduction of salivary 8-oxo-dG by 20 min physical activity. We conclude that the 20 min extensive physical activity increases the level of salivary 8-oxo-dG in healthy donors and 100 ml daily intake of tomato juice may inhibit the increase of 8-oxo-dG in saliva.
Keyphrases
- physical activity
- oxidative stress
- heart rate
- reactive oxygen species
- dna damage
- body mass index
- weight gain
- immune response
- healthcare
- blood pressure
- sleep quality
- cell therapy
- induced apoptosis
- depressive symptoms
- signaling pathway
- diabetic rats
- toll like receptor
- high intensity
- mesenchymal stem cells
- bone marrow
- molecularly imprinted
- stress induced
- solid phase extraction