Ecklonia cava Ameliorates Cognitive Impairment on Amyloid β-Induced Neurotoxicity by Modulating Oxidative Stress and Synaptic Function in Institute of Cancer Research (ICR) Mice.
Hyo-Lim LeeMin Ji GoHan Su LeeHo-Jin HeoPublished in: Antioxidants (Basel, Switzerland) (2024)
This study investigated the neuroprotective effect of 70% ethanol extract of Ecklonia cava (EE) in amyloid beta (Aβ)-induced cognitive deficit mice. As a result of analyzing the bioactive compounds in EE, nine compounds were identified using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). In particular, the diekcol content was quantified by high-performance liquid chromatography with diode-array detection (DAD-HPLC). Biochemical analysis was performed on brain tissue to determine the mechanism of the cognitive function improvement effect of EE. The result showed that EE ameliorated learning and memory decline in behavioral tests on Aβ-induced mice. EE also attenuated oxidative stress by regulating malondialdehyde (MDA) content, reduced glutathione (GSH), and superoxide dismutase (SOD) levels. Similarly, EE also improved mitochondrial dysfunction as mitochondrial membrane potential, ATP production, and reactive oxygen species (ROS) levels. In addition, EE enhanced synapse function by modulating acetylcholine-related enzymes and synaptic structural proteins in the whole brain, hippocampus, and cerebral cortex tissues. Also, EE regulated Aβ-induced apoptosis and inflammation through the c-Jun N-terminal kinase (JNK) and nuclear factor-kappa B (NF-κB) signaling pathways. Furthermore, EE protected neurotoxicity by increasing brain-derived neurotrophic factor (BDNF) production. These results suggest that EE may be used as a dietary supplement for the prevention and treatment of Alzheimer's disease (AD).
Keyphrases
- oxidative stress
- induced apoptosis
- diabetic rats
- signaling pathway
- nuclear factor
- high performance liquid chromatography
- simultaneous determination
- tandem mass spectrometry
- mass spectrometry
- liquid chromatography
- dna damage
- reactive oxygen species
- cognitive impairment
- toll like receptor
- high glucose
- ischemia reperfusion injury
- endoplasmic reticulum stress
- cerebral ischemia
- cell death
- ms ms
- gene expression
- high resolution
- drug induced
- solid phase extraction
- resting state
- white matter
- high fat diet induced
- single cell
- functional connectivity
- stress induced
- pi k akt
- gas chromatography
- hydrogen peroxide
- immune response
- anti inflammatory
- type diabetes
- heat stress
- amyotrophic lateral sclerosis
- prefrontal cortex
- skeletal muscle
- mouse model
- human health
- cognitive decline
- breast cancer cells
- high throughput
- mild cognitive impairment
- heat shock