Thefruits of Gleditsia sinensis Lam. have been utilized to treat inflammatory diseases in China. Echinocystic acid (EA), one pentacyclic triterpenoid isolated from thefruits of G. sinensis, exhibits an anti-inflammatory effect. However, its anti-sepsis activity and mechanism of action, especially the protective effect against sepsis-associated acute kidney injury (SA-AKI), are not investigated yet. This study is to explore the efficacy and potential mechanism of EA on SA-AKI. EA elevated the function of multiple organs and effectively reduced the increased inflammation and apoptosis of kidney tissue and HK-2 cells. DARTS, CETSA, and molecular docking experiments revealed that EA could directly bind to protein tyrosine phosphatase 1B (PTP1B), a widespread prototype non-receptor tyrosine phosphatase. Collectively, EA can alleviate murine SA-AKI though restraining inflammation and apoptosis and may be a potential natural drug for remedying SA-AKI.
Keyphrases
- acute kidney injury
- oxidative stress
- cell cycle arrest
- induced apoptosis
- molecular docking
- cardiac surgery
- cell death
- endoplasmic reticulum stress
- pi k akt
- anti inflammatory
- molecular dynamics simulations
- protein kinase
- binding protein
- amino acid
- human health
- intensive care unit
- signaling pathway
- single cell
- mass spectrometry
- atomic force microscopy
- drug induced
- single molecule