Carbon dioxide expanded liquid: an effective solvent for the extraction of quercetin from South African medicinal plants.
Veronika PilařováLukáš KudaHana Kočová VlčkováLucie NovákováShubhpriya GuptaManoj KulkarniFrantišek ŠvecJohannes Van StadenKarel DoležalPublished in: Plant methods (2022)
The optimized procedure enabled very fast extraction in 30 min using environmentally friendly solvents and it was successfully applied to 16 different plant samples, including 14 bulbs and 2 fruits from South Africa. The quercetin content in extracts was quantified using ultra-high performance liquid chromatography (UHPLC) with tandem mass spectrometry. UHPLC hyphenated with high-resolution mass spectrometry was used to confirm chemical identity of quercetin in the analyzed samples. We quantified quercetin in 11 samples of all 16 tested plants. The quercetin was found in Agapanthus praecox from the Amaryllidaceae family and its presence in this specie was reported for the first time.
Keyphrases
- ultra high performance liquid chromatography
- high resolution mass spectrometry
- tandem mass spectrometry
- liquid chromatography
- gas chromatography
- simultaneous determination
- high performance liquid chromatography
- mass spectrometry
- carbon dioxide
- south africa
- ms ms
- solid phase extraction
- ionic liquid
- high resolution
- hepatitis c virus
- hiv positive
- antiretroviral therapy