Role of reactive oxygen species in the modulation of auxin flux and root development in Arabidopsis thaliana.
Taras P PasternakKlaus PalmeJosé Manuel Pérez-PérezPublished in: The Plant journal : for cell and molecular biology (2023)
Reactive oxygen species (ROS) play a dual role in plant biology, acting as important signal transduction molecules and as toxic byproducts of aerobic metabolism that accumulate in cells upon exposure to different stressors and lead to cell death. In plants, root architecture is regulated by the distribution and intercellular flow of the phytohormone auxin. In this study, we identified ROS as an important modulator of auxin distribution and response in the root. ROS production is necessary for root growth, proper tissue patterning, cell growth, and lateral root (LR) induction. Alterations in ROS balance led to altered auxin distribution and response in SOD and RHD2 loss-of-function mutants. Treatment of Arabidopsis seedlings with additional sources of ROS (hydrogen peroxide) or an ROS production inhibitor (diphenylene iodonium) induced phenocopies of the mutants studied. Simultaneous application of auxin and ROS increased LR primordia induction, and PIN-FORMED protein immunolocalization further demonstrated the existing link between auxin and ROS in orchestrating cell division and auxin flux during root development.
Keyphrases
- reactive oxygen species
- arabidopsis thaliana
- cell death
- dna damage
- cell cycle arrest
- hydrogen peroxide
- stem cells
- transcription factor
- minimally invasive
- nitric oxide
- oxidative stress
- induced apoptosis
- cell proliferation
- bone marrow
- mesenchymal stem cells
- drug induced
- small molecule
- high glucose
- signaling pathway
- endothelial cells
- combination therapy
- diabetic rats
- smoking cessation
- wild type
- binding protein
- cell adhesion