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High-throughput volumetric mapping of synaptic transmission.

Wei ChenXinxin GeQinrong ZhangRyan G NatanJiang Lan FanMassimo ScanzianiNa Ji
Published in: Nature methods (2024)
Volumetric imaging of synaptic transmission in vivo requires high spatial and high temporal resolution. Shaping the wavefront of two-photon fluorescence excitation light, we developed Bessel-droplet foci for high-contrast and high-resolution volumetric imaging of synapses. Applying our method to imaging glutamate release, we demonstrated high-throughput mapping of excitatory inputs at >1,000 synapses per volume and >500 dendritic spines per neuron in vivo and unveiled previously unseen features of functional synaptic organization in the mouse primary visual cortex.
Keyphrases
  • high resolution
  • high throughput
  • single cell
  • mass spectrometry
  • magnetic resonance
  • single molecule
  • high speed
  • prefrontal cortex
  • tandem mass spectrometry
  • computed tomography
  • energy transfer