Angiogenesis Potential of Bladder Acellular Matrix Hydrogel by Compounding Endothelial Cells.

Rapid vascularization is very important in tissue engineering. Bladder acellular matrix (BAM) with inherent bioactive factors, a natural extracellular matrix (ECM) derived biomaterial, has been widely used as a scaffold to facilitate the repair and reconstruction of urinary tissues. However, the application of the traditional BAM scaffold has been limited due to the dense structure. To investigate the angiogenic potential of BAM, BAM hydrogels with tailored porous structures were prepared in this study by tuning BAM concentrations (4, 6, and 8 mg/mL). The 6 mg/mL BAM hydrogel was loaded with porcine iliac endothelial cells (PIECs), and their angiogenic potential was analyzed in vitro and in vivo . The mechanical strength and gelation speed of the BAM hydrogels increased, while their pore size decreased as concentration increased. Commercially available collagen hydrogel (2.5 mg/mL) showed weaker mechanical properties than BAM hydrogels but similar gelation speed and pore size as 6 mg/mL BAM hydrogel. To ensure a similar three-dimensional microenvironment for the PIECs, 6 mg/mL BAM and collagen hydrogels were selected for the in vitro and in vivo experiments. A significantly higher density of viable, fusiform PIECs of average length ∼50 μm was observed in the BAM hydrogel, while those inside the collagen hydrogel were spherical and ∼30 μm long. In addition, the PIECs/BAM hydrogel resulted in significantly higher revascularization compared with the PIECs/collagen and unloaded BAM hydrogels. The higher angiogenic potential of the PIECs/BAM hydrogel is due to the growth factors that promote PIEC proliferation and therefore vascularization.