Identification of nontuberculous mycobacteria using the GenoType CMdirect and GenoType Mycobacterium CM line probe assays in GeneXpert MTB/RIF Ultra negative sputum: A South African prevalence study.

Christoffel OppermanChaldene JohannesSarishna SinghYonas GhebrekristosRob WarrenAliasgar EsmailWynand Goosen

Published in: Diagnostic microbiology and infectious disease (2024)

The GenoType CMdirect Ver 1.0 and GenoType Mycobacterium Common Mycobacteria Ver 2.0-line probe assays were utilized to identify nontuberculous mycobacteria (NTM) from decontaminated sputum in a GeneXpert MTB/RIF Ultra negative cohort. A preliminary NTM prevalence of 1.7 % was observed, with a 100 % specificity. The high specificity advocates the CMdirect as a possible rule-in test for NTM disease.

Keyphrases

pulmonary tuberculosis
mycobacterium tuberculosis
risk factors
high throughput
high resolution
cystic fibrosis
quantum dots
living cells
mass spectrometry
fluorescent probe