Self-Guiding Polymeric Prodrug Micelles with Two Aggregation-Induced Emission Photosensitizers for Enhanced Chemo-Photodynamic Therapy.
Xiaoqing YiJing-Jing HuJun DaiXiaoding LouZhujin ZhaoFan XiaBen-Zhong TangPublished in: ACS nano (2021)
Nowadays, aggregation-induced emission luminogens (AIEgens) with reactive oxygen species (ROS) generating ability have been used as photosensitizers for imaging guided photodynamic therapy (PDT). To achieve enhanced antitumor outcomes, combining AIEgens-based PDT with chemotherapy is an efficient strategy. However, the therapeutic efficiency is hampered by the limited cellular uptake efficiency and the appropriate light irradiation occasion. In this paper, a self-guiding polymeric micelle (TB@PMPT) composed of two AIE photosensitizers and a reduction-sensitive paclitaxel prodrug (PTX-SS-N3) was established for enhanced chemo-photodynamic therapy by a dual-stage light irradiation strategy. When the micelles were accumulated in tumor tissues, the first light irradiation (L1, 6 min) was utilized to facilitate cellular uptake by "photochemical internalization" (PCI). Then, the intracellular glutathione (GSH) would induce the PTX release, micelles disassembly and the aggregation state change of AIEgens. The fluorescence signal change of two AIEgens-based ratiometric fluorescent probe could not only precisely guide the second light irradiation (L2, 18 min) for sufficient ROS production, but also monitor the nonfluorescent drug PTX release in turn. Both in vivo and in vitro studies demonstrated that the dual-stage light irradiation strategy employed for TB@PMPT micelles exhibited a superior therapeutic effect over only 24 min continuous light irradiation.
Keyphrases
- photodynamic therapy
- fluorescent probe
- cancer therapy
- drug delivery
- drug release
- living cells
- fluorescence imaging
- reactive oxygen species
- radiation induced
- mycobacterium tuberculosis
- dna damage
- single molecule
- cell death
- emergency department
- gene expression
- type diabetes
- acute coronary syndrome
- high resolution
- sensitive detection
- mass spectrometry
- metabolic syndrome
- quantum dots
- insulin resistance
- skeletal muscle
- weight loss
- antiplatelet therapy
- electronic health record