Haplotype analysis of VEGF gene polymorphisms in polycystic ovary syndrome.
Mariana Kefalás de Oliveira GomesDeize de Cássia AntoninoMarly Aparecida Spadotto BalarinSarah Cristina Sato Vaz TanakaMarina Almeida CaldeiraAlessandra Bernadete Trovó de MarquiMarco Fábio Prata LimaElisabete Aparecida Mantovani ResendeMariangela Torreglosa Ruiz CintraPublished in: Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology (2019)
This study evaluated the association of polymorphisms of VEGF (endothelial vascular growth factor) gene + 936C/T (rs3025039), 1154 G/A (rs 1570360) and -2578 C/A (rs 699947) in patients with polycystic ovary syndrome (PCOS) and to perform the haplotypes formed by the alleles in the Brazilian population. A total of 110 women without PCOS and 112 women with PCOS were included in the study. Genotyping analyses were performed using the PCR-RFLP assays (rs 3025039 and rs 699947) and by allelic discrimination using the real-time PCR technique (rs 1570360). In the univariate analysis, we observed a significant difference between the groups for the polymorphism rs 1570360 and this polymorphism presented statistical differences between the groups for the recessive model (p = .04). The frequency of the T-G-C haplotype showed a statistically significant difference between women with PCOS and controls (p = .05). The -2578 A/C polymorphism was more frequent in the control group, which may be associated with a protective characteristic for the PCOS manifestation. In the sample analysis, polymorphism rs 1570360 is associated with PCOS and the T-G-C haplotype could be associated with protective factors.