Design and optimization of an improved qPCR assay for the detection of Histoplasma capsulatum.

Bianca Kelly Neves Izidro da SilvaAna Cláudia Alves CortezLuciana Aires OliveiraJuan Diego Ribeiro de AlmeidaÉrica Simplício de SouzaKatia Santana CruzAni Beatriz Jackisch MatsuuraMauricio Morishi OguskuKarolina Jeaneth Solórzano ChavarríaDjane Baía-da-SilvaQuique BassatMarcus Vinícius Guimarães LacerdaHagen FrickmannJoão Vicente Braga de Souza

Published in: European journal of microbiology & immunology (2023)

The improved qPCR assessed in this study was successful in detecting H. capsulatum with high efficiency and accuracy in in-vitro evaluation, including the identification of the target pathogen in both clinical and environmental samples.

Keyphrases

high efficiency
human health
candida albicans
real time pcr
risk assessment
climate change
life cycle