Identification of ALP+/CD73+ defining markers for enhanced osteogenic potential in human adipose-derived mesenchymal stromal cells by mass cytometry.

Daisy D CanepaElisa A CasanovaEirini ArvanitiVinko TosevskiSonja MärsmannBenjamin EggerschwilerSascha HalvachizadehJohanna BuschmannAndré A BarthJan A PlockManfred ClaassenHans-Christoph PapePaolo Cinelli

Published in: Stem cell research & therapy (2021)

The data obtained reveal, at single-cell level, the heterogeneity of AD-MSCs from several donors and highlight how cellular composition impacts the osteogenic differentiation capacity. The marker combination (ALP/CD73) can not only be used to assess the differentiation potential of undifferentiated AD-MSC preparations, but also could be employed to prospectively enrich AD-MSCs from the stromal vascular fraction of human adipose tissue for therapeutic applications.

Keyphrases

single cell
mesenchymal stem cells
bone marrow
endothelial cells
adipose tissue
rna seq
umbilical cord
induced pluripotent stem cells
high throughput
high fat diet
insulin resistance
gene expression
electronic health record
metabolic syndrome
dna methylation