A Nano-Electroporation-DNA Tensioner Platform Enhances Intracellular Delivery and Mechanical Analysis Toward Rapid Drug Assessment.
Xinxin HangZhaocun HuangShiqi HeZhiying WangZaizai DongLingqian ChangPublished in: Small methods (2023)
In vitro, drug assessment holds tremendous potential to success in novel drug development and precision medicine. Traditional techniques for drug assessment, however, face remarkable challenges to achieve high speed, as limited by incubation-based drug delivery (>several hours) and cell viability measurements (>1 d), which significantly compromise the efficacy in clinical trials. In this work, a nano-electroporation-DNA tensioner platform is reported that shortens the time of drug delivery to less than 3 s, and that of cellular mechanical force analysis to 30 min. The platform adopts a nanochannel structure to localize a safe electric field for cell perforation, while enhancing delivery speed by 10 3 times for intracellular delivery, as compared to molecular diffusion in coculture methods. The platform is further equipped with a DNA tensioner to detect cellular mechanical force for quantifying cell viability after drug treatment. Systematic head-to-head comparison, by analyzing FDA (food and drug administration)-approved drugs (paclitaxel, doxorubicin), demonstrated the platform with high speed, efficiency, and safety, showing a simple yet powerful tool for clinical drug screening and development.
Keyphrases
- high speed
- drug delivery
- single molecule
- high throughput
- atomic force microscopy
- clinical trial
- drug administration
- cancer therapy
- adverse drug
- drug induced
- circulating tumor
- emergency department
- stem cells
- high resolution
- cell free
- mesenchymal stem cells
- study protocol
- sensitive detection
- circulating tumor cells
- phase iii