Large-scale phenotyping of ABR P1-N1 amplitudes before and after exposure to noise in 69 strains of mice.
Joel LavinskyAline MendonçaMariele BressanVagner Antonio Rodrigues da SilvaGuilherme KasperbauerJuemei WangPezhman SalehiEly Cheikh BoussatyRick Adam FriedmanPublished in: Mammalian genome : official journal of the International Mammalian Genome Society (2021)
ABR wave I amplitude represents the synapse of auditory nerve fibers with the inner hair cell and is highly correlated with synapse counts. Cochlear synaptopathy, the loss of synaptic connections between inner hair cells and auditory nerve fibers, has been well-demonstrated in animal models of noise-induced hearing loss. The peak-to-peak wave I amplitude was determined at baseline and 2 weeks after noise exposure. We determined the ABR wave I amplitude at 80 dB SPL at the frequencies of 8, 12, 16, 24, and 32 kHz. A total of 69 strains (1-8 mice/strain) were analyzed. A statistically significant post-noise reduction in wave I amplitude was observed in all the tested frequencies (p < 0.00001). We identify distinct patterns of noise susceptibility and make this complete phenotypic dataset available for general use. This data establishes a new resource for the study of NIHL in mice and we hope this database will be a useful tool to expand the research in this field.
Keyphrases
- hearing loss
- air pollution
- high fat diet induced
- resting state
- escherichia coli
- induced apoptosis
- working memory
- high throughput
- cell therapy
- single cell
- oxidative stress
- bone marrow
- electronic health record
- stem cells
- high glucose
- metabolic syndrome
- endoplasmic reticulum stress
- mesenchymal stem cells
- diabetic rats
- adverse drug
- deep learning
- cell proliferation
- signaling pathway
- endothelial cells
- pi k akt