Protein Kinase CK2 Contributes to Glucose Homeostasis by Targeting Fructose-1,6-Bisphosphatase 1.
Mandy PackTim Nikolai GuldeMichelle Victoria VölckerAnne S BoeweSelina WrublewskyEmmanuel AmpofoMathias MontenarhClaudia GötzPublished in: International journal of molecular sciences (2022)
Glucose homeostasis is of critical importance for the survival of organisms. It is under hormonal control and often coordinated by the action of kinases and phosphatases. We have previously shown that CK2 regulates insulin production and secretion in pancreatic β-cells. In order to shed more light on the CK2-regulated network of glucose homeostasis, in the present study, a qRT-PCR array was carried out with 84 diabetes-associated genes. After inhibition of CK2, fructose-1,6-bisphosphatase 1 (FBP1) showed a significant lower gene expression. Moreover, FBP1 activity was down-regulated. Being a central enzyme of gluconeogenesis, the secretion of glucose was decreased as well. Thus, FBP1 is a new factor in the CK2-regulated network implicated in carbohydrate metabolism control.
Keyphrases
- protein kinase
- gene expression
- blood glucose
- type diabetes
- transcription factor
- glycemic control
- cardiovascular disease
- induced apoptosis
- dna methylation
- high resolution
- cell cycle arrest
- cell proliferation
- blood pressure
- oxidative stress
- mass spectrometry
- network analysis
- free survival
- bioinformatics analysis
- real time pcr