False detection of rifampicin resistance using Xpert® MTB/RIF Ultra assay due to an A451V mutation in Mycobacterium tuberculosis.

Margaret M FitzgibbonEmma RoycroftGerard SheehanAnne-Marie Mc LaughlinKeith Ian QuintyneElaine BrabazonMary O'MearaPeter R FlanaganA-Louise SeagarIan F LaurensonJoseph KeaneThomas R Rogers

Published in: JAC-antimicrobial resistance (2021)

False detection of rifampicin resistance, albeit rare, should be considered possible with Xpert® MTB/RIF Ultra assay, particularly in low TB incidence settings. Confirmatory sequencing methods should be performed to prevent the unnecessary use of second-line anti-tuberculous drugs.

Keyphrases

mycobacterium tuberculosis
pulmonary tuberculosis
high throughput
loop mediated isothermal amplification
high resolution
label free
real time pcr
risk factors
single cell
mass spectrometry