Nerve growth factor loaded macrophage-derived nanovesicles for inhibiting neuronal apoptosis after spinal cord injury.
Nan XiaZhanshan GaoHengshuo HuDaoyong LiChuanjie ZhangXifan MeiChao WuPublished in: Journal of biomaterials applications (2021)
Spinal cord injury (SCI) is an extremely destructive central nervous system lesion. Studies have shown that NGF can promote nerve regeneration after SCI. However, it cannot produce the desired effect due to its stability in the body and is difficulty in passing through the blood-brain barrier. In this study, we prepared nanovesicles derived from macrophage membrane encapsulating NGF (NGF-NVs) as a drug carrier for the treatment of SCI. Cell experiments showed that NGF-NVs were effectively taken up by PC12 cells and inhibited neuronal apoptosis. In vivo imaging experiments, a large quantity of NGF was delivered to the injured site with the aid of the good targeting of NVs. In animal experiments, NGF-NVs improved the survival of neurons by significantly activating the PI3K/AKT signaling pathway and had good behavioral and histological recovery effects after SCI. Therefore, NVs are a potential drug delivery vector for SCI therapy.
Keyphrases
- growth factor
- spinal cord injury
- signaling pathway
- drug delivery
- spinal cord
- neuropathic pain
- cancer therapy
- oxidative stress
- endoplasmic reticulum stress
- adipose tissue
- cell cycle arrest
- epithelial mesenchymal transition
- high resolution
- cell death
- emergency department
- cerebral ischemia
- bone marrow
- cell proliferation
- risk assessment
- photodynamic therapy
- free survival
- blood brain barrier
- replacement therapy
- brain injury
- mass spectrometry
- cerebrospinal fluid
- single cell
- drug induced
- combination therapy
- subarachnoid hemorrhage
- wound healing
- climate change