Hi-C techniques: from genome assemblies to transcription regulation.

The invention of chromosome-conformation capture (3C) techniques, in particular the key method Hi-C providing genome-wide information about chromatin contacts, revolutionized the way we study the three-dimensional (3D) organization of the nuclear genome and how it impacts transcription, replication and DNA repair. Since the frequency of chromatin contacts between pairs of genomic segments predictably relates to the distance in the linear genome, the Hi-C information has also proved useful for scaffolding genomic sequences. Here, we review recent enhancements in experimental procedures of Hi-C and its various derivatives such as Micro-C, HiChIP, and Capture Hi-C. We assess advantages and limitations of the techniques, and present examples of their use in recent plant studies. We also report on progress in computational tools used in assembling genome sequences.