Anti-inflammatory Lathyrane Diterpenoids from Euphorbia lathyris.
Cui-Yun ZhangYan-Li WuPeng ZhangZhuang-Zhuang ChenHua LiLi-Xia ChenPublished in: Journal of natural products (2019)
Six new lathyrane diterpenoids (1-6) and 10 known analogues (7-16), were separated from the seeds of Euphorbia lathyris. The absolute configuration of 1 was determined by X-ray crystallography, and the C-2' configuration of 5 was elucidated by comparing experimental and calculated ECD data. These compounds were studied for their inhibition against nitric oxide (NO) generation induced by lipopolysaccharide in RAW264.7 macrophage cells. Compounds 1-3, 7, 9, 11, 13, 14, and 16 displayed inhibitory effects on NO production, with IC50 values of 2.6-26.0 μM. The new compound 1 (IC50 3.0 ± 1.1 μM), with no obvious cytotoxicity, was selected for further experiments. The production of cytokines such as IL-6 and IL-1β, as well as the protein expression of iNOS, NF-κB, and phosphorylated IκBα, was reduced by 1 dose-dependently. These results suggested that lathyrane diterpenoids may be used as potential anti-inflammatory agents and are worth being further researched.
Keyphrases
- anti inflammatory
- nitric oxide
- induced apoptosis
- lps induced
- nitric oxide synthase
- signaling pathway
- cell cycle arrest
- inflammatory response
- oxidative stress
- high resolution
- electronic health record
- adipose tissue
- pi k akt
- hydrogen peroxide
- magnetic resonance imaging
- endoplasmic reticulum stress
- big data
- cell death
- immune response
- cell proliferation
- human health
- dual energy
- climate change
- data analysis
- magnetic resonance