Alternative splicing of the receptor-like kinase Nt-Sd-RLK in tobacco cells responding to lipopolysaccharides: suggestive of a role in pathogen surveillance and perception?
Natasha M SanabriaIan A DuberyPublished in: FEBS letters (2016)
Nt-Sd-RLK encodes an S-domain lectin receptor-like kinase that is induced in response to microbe-associated molecular pattern molecules (MAMPs) such as lipopolysaccharide (LPS). In this study, we investigated the alternative splicing of Nt-Sd-RLK in response to LPS stimulation. Our data indicate that in nonstimulated cells, a shorter transcript of Nt-Sd-RLK is generated and that in response to LPS, alternative splicing produces the full-length transcript. We propose that the extracellular domain of Nt-Sd-RLK encoded by the shorter transcript functions in pathogen surveillance. Once this domain binds LPS, alternative splicing generates the kinase domain-containing Nt-Sd-RLK that activates downstream signalling leading to a defence response. Thus, our findings suggest that plant defence signalling may be regulated through the alternative splicing of receptor-like kinases involved in pathogen recognition.
Keyphrases
- inflammatory response
- induced apoptosis
- anti inflammatory
- cell cycle arrest
- public health
- candida albicans
- rna seq
- protein kinase
- toll like receptor
- lps induced
- electronic health record
- machine learning
- single cell
- cell death
- mass spectrometry
- immune response
- big data
- artificial intelligence
- endothelial cells
- data analysis
- drug induced
- high speed