Sequential Single-Cell Transcriptional and Protein Marker Profiling Reveals TIGIT as a Marker of CD19 CAR-T Cell Dysfunction in Patients with Non-Hodgkin Lymphoma.

Zachary JacksonChangjin HongRobert Schauner Boro DropulićPaolo F CaimiMarcos J deLima Maria Florencia GiraudoKalpana GuptaJane S ReeseTae Hyun Hwang David N Wald

Published in: Cancer discovery (2022)

This is the first study investigating the mechanisms linked to CAR-T patient responses based on the sequential analysis of manufactured and infused CAR-T cells using single-cell RNA and protein expression data. Furthermore, our findings are the first to demonstrate an improvement of CAR-T cell efficacy with TIGIT inhibition alone. This article is highlighted in the In This Issue feature, p. 1825.

Keyphrases

single cell
rna seq
high throughput
gene expression
machine learning
oxidative stress
case report
transcription factor
big data
small molecule
binding protein
nucleic acid
heat shock
neural network