Effects of cell seeding density on real-time monitoring of anti-proliferative effects of transient gene silencing.
Cigdem SelliYasemin EracMetiner TosunPublished in: Journal of biological research (Thessalonike, Greece) (2016)
Data suggest that the effects of transient silencing on cell proliferation differ depending on the initial cell seeding density. While high seeding densities mask the significant changes in proliferation, the inhibitory effects of silencing become apparent at lower seeding densities as the entry into log phase is delayed. Using the optimal initial seeding density is crucial when studying the effects of transient gene silencing. In addition, the results suggest that TRPC1 may contribute to proliferation and phenotypic switching of vascular smooth muscle cells.
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