Antimycobacterial and PknB Inhibitory Activities of Venezuelan Medicinal Plants.
Carlos AranagaS FraileA TorresA FalcoF MichelangeliH TakiffPublished in: International journal of microbiology (2020)
Global control and elimination of tuberculosis are hindered by the high prevalence of drug-resistant strains, making the development of new drugs to fight tuberculosis a public health priority. In this study, we evaluated 118 extracts from 58 Venezuelan plant species for their ability to inhibit the growth of Mycobacterium tuberculosis mc26020, using the agar dilution method. Additionally, we determined the ability of these extracts to inhibit the activity of PknB protein, an essential M. tuberculosis serine/threonine kinase, using a high-throughput luminescent assay. Of the 118 extracts tested, 14 inhibited bacterial growth with a minimum inhibitory concentration ≤500 μg/ml, and 36 inhibited the kinase activity with a half-maximal inhibitory concentration <200 μg/ml. Five extracts inhibited M. tuberculosis growth and inhibited the activity of the kinase protein, suggesting that this could be the basis of their growth inhibition.
Keyphrases
- mycobacterium tuberculosis
- drug resistant
- high throughput
- public health
- protein kinase
- pulmonary tuberculosis
- multidrug resistant
- hiv aids
- escherichia coli
- tyrosine kinase
- protein protein
- blood pressure
- pseudomonas aeruginosa
- ms ms
- liquid chromatography tandem mass spectrometry
- body composition
- electronic health record
- energy transfer