Neuroprotective effect of rutin against colistin-induced oxidative stress, inflammation and apoptosis in rat brain associated with the CREB/BDNF expressions.
Hamit ÇelikFatih Mehmet KandemirCuneyt CaglayanSelçuk ÖzdemirSelim ÇomaklıSefa KucuklerAhmet YardımPublished in: Molecular biology reports (2020)
The purpose of the current study was to examine the neuroprotective effect of rutin against colistin-induced neurotoxicity in rats. Thirty-five male Sprague Dawley rats were randomly divided into 5 groups. The control group (orally received physiological saline), the rutin group (orally administered 100 mg/kg body weight), the colistin group (i.p. administered 15 mg/kg body weight), the Col + Rut 50 group (i.p. administered 15 mg/kg body weight of colistin, and orally received 50 mg/kg body weight of rutin), the Col + Rut 100 group (i.p. administered 15 mg/kg body weight of colistin, and orally received 100 mg/kg body weight of rutin). Administration of colistin increased levels of glial fibrillary acidic protein and brain-derived neurotrophic factor and acetylcholinesterase and butyrylcholinesterase activities while decreasing level of cyclic AMP response element binding protein and extracellular signal regulated kinases 1 and 2 (ERK1/2) expressions. Colistin increased oxidative impairments as evidenced by a decrease in level of nuclear factor erythroid 2-related factor 2 (Nrf-2), glutathione, superoxide dismutase, glutathione peroxidase and catalase activities, and increased malondialdehyde content. Colistin also increased the levels of the apoptotic and inflammatoric parameters such as cysteine aspartate specific protease-3 (caspase-3), p53, B-cell lymphoma-2 (Bcl-2), nuclear factor kappa B (NF-κB), Bcl-2 associated X protein (Bax), tumor necrosis factor-α (TNF-α) and neuronal nitric oxide synthase (nNOS). Rutin treatment restored the brain function by attenuating colistin-induced oxidative stress, apoptosis, inflammation, histopathological and immunohistochemical alteration suggesting that rutin supplementation mitigated colistin-induced neurotoxicity in male rats.
Keyphrases
- body weight
- acinetobacter baumannii
- escherichia coli
- klebsiella pneumoniae
- nuclear factor
- pseudomonas aeruginosa
- gram negative
- multidrug resistant
- drug resistant
- oxidative stress
- toll like receptor
- nitric oxide synthase
- cystic fibrosis
- binding protein
- hydrogen peroxide
- rheumatoid arthritis
- signaling pathway
- nitric oxide
- endoplasmic reticulum stress
- induced apoptosis
- cerebral ischemia
- immune response
- transcription factor
- small molecule
- subarachnoid hemorrhage
- pi k akt
- drug induced
- brain injury
- diffuse large b cell lymphoma
- white matter
- lps induced
- spinal cord
- single molecule