Preparation and application of chlorpyrifos molecularly imprinted solid-phase microextraction probes for the residual determination of organophosphorous pesticides in fresh and dry foods.

A solid-phase microextraction probe was prepared on the surface of a stainless-steel wire through molecular sol-gel imprinting technology using chlorpyrifos as a template molecule, tetraethoxysilane as a sol-gel precursor, and acrylamide and β-cyclodextrin as functional monomers. The polymer was characterized by infrared spectrometry and scanning electron microscopy. Moreover, the selectivity and the parameters including the type and volume of the extraction solvents, ionic strength, pH, temperature, extraction time, stirring speed, and desorption time affecting extraction performance were evaluated. Under the optimum solid-phase microextraction and gas chromatography conditions, the linear ranges were 0.25-25.0 μg/L for chlorpyrifos, quinalphos, triazophos, pirimiphos-methyl, and chlorpyrifos-methyl with the correlation coefficient above 0.99. The detection limits (S/N = 3) were in the range of 0.02-0.07 μg/L and the RSDs were <7.3%. The developed method was successfully used to determine the multi-residues of chlorpyrifos, quinalphos, triazophos, pirimiphos-methyl, and chlorpyrifos-methyl in green peppers and cinnamon with satisfactory recoveries.