Direct Identification of Disaccharide Structural Isomers Using Ambient Ionization Tandem Mass Spectrometry with In Situ Methylation.
Rongfan RenMinghui YuanHongli LiDavid Da Yong ChenPublished in: Analytical chemistry (2023)
Carbohydrates play critically important roles in energy supply and biological functions in living systems. However, it has been a great challenge to identify saccharides and distinguish their isomers because they have highly similar structures and many possible positions for glycosidic linkages. In this work, an ambient ionization tandem mass spectrometry method was developed to characterize disaccharide structural isomers with in situ methylation. The direct analysis in real time ion source can be used to facilitate the methylation reaction of disaccharides with tetramethylammonium hydroxide. The hydroxyl groups of disaccharides can be methylated instantaneously, and the products can be ionized at the same time. The methylated product ions from full scan mass spectrometry (MS) and tandem MS can be used to distinguish a variety of disaccharide structural isomers with different glycosidic linkages, compositions, and configurations. Characteristic marker ions were discovered, and they can be used for the assignment of linkage type and identification of specific isomeric forms. The method was used for the direct identification of disaccharide isomers from real commercial products such as honey, wine, and milk without complex sample pretreatment or chromatographic separation.
Keyphrases
- mass spectrometry
- tandem mass spectrometry
- gas chromatography
- liquid chromatography
- ultra high performance liquid chromatography
- high performance liquid chromatography
- simultaneous determination
- high resolution mass spectrometry
- high resolution
- genome wide
- solid phase extraction
- air pollution
- dna methylation
- capillary electrophoresis
- particulate matter
- bioinformatics analysis
- ms ms
- computed tomography
- multiple sclerosis
- quantum dots
- water soluble
- gold nanoparticles
- human immunodeficiency virus
- hiv infected
- hepatitis c virus