Optimization of Blood Handling and Peripheral Blood Mononuclear Cell Cryopreservation of Low Cell Number Samples.
Christopher M HopeDao HuynhYing Ying WongHelena OakeyGriffith Boord PerkinsTrung NguyenSabrina BinkowskiMinh BuiAce Y L ChooEmily GibsonDexing HuangKi Wook KimKatrina NguiWilliam D RawlinsonTimothy SadlonJennifer J CouperMegan A S PennoSimon C Barrynull On Behalf Of The Endia Study GroupPublished in: International journal of molecular sciences (2021)
A sample delay of 24 h at RT does not impact the viability and total viable cell numbers. When long-term delays exist (>4 d) total viable cell number and cell viability losses are reduced in samples stored at 4 °C. Immune phenotype and function are slightly altered after 24 h of storage, further impacts of storage are reduced in samples stored at 4 °C.