On the synergy of biomicrofluidic technologies and real-time 3D tracking: A perspective.

Particle image velocimetry and particle tracking velocimetry have played pivotal roles in flow and particle characterization, owing to their non-invasive and accurate data collection methods. However, their broader application in the biomicrofluidics field is constrained by challenges, such as intensive calibration, high post-processing costs, and optical compatibility issues, especially in settings where space is a bottleneck. This article describes recent advancements in non-iterative ray tracing that promise more streamlined post-capture calibration and highlights examples of applications and areas that merit further technological investigation. The development and adoption of these techniques may pave the way for new innovations.