Distinct chemical composition and enzymatic treatment induced human endothelial cells survival in acellular ovine aortae.
Morteza HeidarzadehReza RahbarghaziShirin SaberianpourAref DelkhoshHassan AminiEmel SokulluMehdi HassanpourPublished in: BMC research notes (2021)
Hematoxylin-Eosin staining showed an appropriate acellularization rate in ovine aortae, indicated by a lack of cell nuclei in the tunica media layer. DAPI staining confirmed the lack of nuclei in the vascular wall after being exposed to the combination of chemical and enzymatic solutions. Verhoeff-Van Gieson staining showed that elastin fibers were diminished in acellular samples compared to the control group while collagen stands were unchanged. CCK-8 survival assay showed enhanced viability in human umbilical vein endothelial cells 5 days after being cultured on decellularized samples compared to the cells cultured on a plastic surface (p < 0.05). SEM imaging showed flattening of endothelial cells on the acellular surface.
Keyphrases
- endothelial cells
- high glucose
- vascular endothelial growth factor
- hydrogen peroxide
- flow cytometry
- induced apoptosis
- breast reconstruction
- high resolution
- free survival
- single cell
- high throughput
- cell cycle arrest
- tissue engineering
- cell death
- cell proliferation
- extracellular matrix
- oxidative stress
- nitric oxide
- mass spectrometry
- combination therapy
- mesenchymal stem cells
- smoking cessation
- endoplasmic reticulum stress
- pi k akt
- drug induced
- wound healing
- stress induced