The blood sample of a pregnant Chinese woman of the north Han population was confirmed as D variation by serologic method, and subsequent detection of RHD Exons 1 through 10 by the polymerase chain reaction-sequence-specific primer test showed that all exons were present. To further clarify the molecular mechanism of this sample, we sequenced the RHD Exons 1 through 10 and found that it was a novel allele caused by c.739 G> C in Exon 5.