Study on Secondary Metabolites of Endophytic Fungus, Aspergillus fumigatus , from Crocus sativus L. Guided byUHPLC-HRMS/MS-Based Molecular Network.

As a traditional Chinese medicine, Crocus sativus Linn has been used for a long time in China. However, the studies on secondary metabolites of its endophytic fungi were not fully sufficient. Thus, the endophytic fungus, Aspergillus fumigatus , collected from the lateral buds of C. sativus , was here investigated. An approach combining UHPLC-HRMS/MS (ultra-high performance liquid chromatography-high resolution mass spectrometry) with molecular network was carried out to construct a molecular network of crude EtOAc extract (CEE) of A. fumigatus , in which 32 chemical compounds were annotated. On the basis of analysis results, a total of 15 known natural compounds were isolated from CEE. Among them, compounds 11 and 12 were isolated for the first time from the genus Aspergillus . Moreover, CEE and compound 7 exhibited moderate inhibitory activity against Erwinia sp. with a MIC value of 100  μ g/mL. This study provided a more convenient and rapid approach to investigating the crude extract with complex components of A. fumigatus , which is of great benefit to the further study and utilization of secondary metabolites of the genus Aspergillus .