Reversible dynamic optical sensing based on coumarin modified β-cyclodextrin for glutathione in living cells.

Zhixue LiuMengdi TianHeng ZhangYu Liu

Published in: Chemical communications (Cambridge, England) (2023)

Coumarin acting as an optical probe was modified on ethylenediamine β-cyclodextrin, which not only enhanced its molecular binding affinity to glutathione (GSH) by a reversible Michael addition, showing 113 times more affinity than that of coumarin itself, but also achieved dynamic real-time sensing of glutathione in living HeLa cells.

Keyphrases

fluorescent probe
living cells
capillary electrophoresis
cell cycle arrest
high resolution
induced apoptosis
high speed
mass spectrometry
ionic liquid
pi k akt
binding protein