Parametric bootstrapping for biological sequence motifs.

Despite the historical centrality of biological sequence motif analysis, this study constitutes to our knowledge the first use of principled null hypotheses for sequence motifs given information content. Through their use, we are able to characterize for the first time differerences in global motif statistics between biological motifs and their null distributions. In particular, we observe that biological sequence motifs show an unusual distribution of IGC, presumably due to biochemical constraints on the mechanisms of direct read-out.