Genetic tool for fate mapping of Oct4 (Pou5f1)-expressing cells and their progeny past the pluripotency stage.
Andrey A KuzminVeronika V ErmakovaSergey A SinenkoSergey V PonomartsevTatiana Y StarkovaElena V SkvortsovaOlga CherepanovaAlexey N TomilinPublished in: Stem cell research & therapy (2019)
The developed O4S system can be used to detect Oct4 activation event, both permanent and transient, in somatic cell types outside the germline. The approach can be equally adjusted to other genes, provided the first component of the system is placed under transcriptional control of these genes, thus, making it a valuable tool for cell fate mapping in mice.
Keyphrases
- cell fate
- genome wide
- high resolution
- optical coherence tomography
- induced apoptosis
- copy number
- diabetic retinopathy
- bioinformatics analysis
- cell cycle arrest
- high density
- dna methylation
- genome wide identification
- single cell
- gene expression
- cell therapy
- transcription factor
- high fat diet induced
- cerebral ischemia
- dna repair
- genome wide analysis
- cell death
- metabolic syndrome
- wild type
- type diabetes
- adipose tissue
- mesenchymal stem cells
- blood brain barrier
- bone marrow
- signaling pathway
- pi k akt
- dna damage