Chlorination-induced p K a decrease: an improved strategy to design ratiometric hypochlorite fluorescent probes with ideally high selectivity.
Shaohui HanBinghui DingHaiqing XiongBojun ZuoYong YeHai-Pu LiXiangzhi SongPublished in: The Analyst (2022)
Based on the selective ClO - -triggered chlorination reaction and the subsequent p K a decrease of phenols, a new strategy was developed for rationally designing ratiometric ClO - fluorescent probes with high selectivity. By investigating the fluorescence responses of 6-cyano-2-naphthol toward ClO - and the p K a -dependent response mechanism, we developed a rapid, sensitive and selective two-photon ratiometric fluorescent probe, Naph-DFOB, to detect ClO - . This probe displayed a ratiometric fluorescence change (from 509 nm to 628 nm) toward ClO - and was successfully applied to image intracellular ClO - in living cells with two-photon excitation. Using Naph-DFOB as a useful tool, the investigation of lipopolysaccharide (LPS)-induced acute lung injury in a mouse model was effectively performed.
Keyphrases
- machine learning
- living cells
- fluorescent probe
- lps induced
- single molecule
- inflammatory response
- deep learning
- mouse model
- energy transfer
- drinking water
- lipopolysaccharide induced
- photodynamic therapy
- toll like receptor
- high glucose
- immune response
- endothelial cells
- diabetic rats
- drug induced
- loop mediated isothermal amplification