SERS determination of dopamine using metal-organic frameworks decorated with Ag/Au noble metal nanoparticle composite after azo derivatization with p-aminothiophenol.
Anni ZhuTiansheng WangYuning JiangSen HuWanxin TangXinling LiuXiaoyu GuoYe YingYiping WuYing WenHai-Feng YangPublished in: Mikrochimica acta (2022)
A specific surface-enhanced Raman scattering (SERS) assay for dopamine (DA) based on an azo derivatization reaction is proposed for the first time by preparation of p-aminothiophenol (PATP)-modified composite SERS substrate, composed of metal-organic framework (MIL-101) decorated with Au and Ag nanoparticles. As the result, the SERS method for detection of the azo reaction between PATP and DA exhibits superior sensitivity, selectivity, and stability. A reasonable linearity in the range 10 -6 to 10 -10 mol∙L -1 is achieved, and the limit of detection is 1.2 × 10 -12 mol∙L -1 . The reactive SERS assay is free from interference in complex physiological fluid. The feasibility of the proposed SERS method for the detection of DA levels in fetal bovine serum (FBS) samples and human serum samples is validated by HPLC-MS methods, displaying promising application potential in early disease diagnosis.
Keyphrases
- sensitive detection
- metal organic framework
- quantum dots
- loop mediated isothermal amplification
- label free
- gold nanoparticles
- ms ms
- reduced graphene oxide
- raman spectroscopy
- solid phase extraction
- simultaneous determination
- high performance liquid chromatography
- liquid chromatography tandem mass spectrometry
- molecularly imprinted
- tandem mass spectrometry
- highly efficient
- mass spectrometry
- high throughput
- real time pcr
- visible light
- uric acid
- multiple sclerosis
- liquid chromatography
- metabolic syndrome
- high resolution
- ultra high performance liquid chromatography
- structural basis
- risk assessment
- gas chromatography
- transition metal