Highly specific PCR-RFLP assays for karyotyping the widespread 2Rb inversion in malaria vectors of the Anopheles gambiae complex.

Raquel Montanez-GonzalezVerena PichlerMaria CalzettaRachel R LoveAlexandra ValleraLydia SchaecherBeniamino CaputoMarco PombiVincenzo PetrarcaAlessandra Della TorreNora J Besansky

Published in: Parasites & vectors (2020)

The availability of highly specific, cost effective and accessible molecular assays for genotyping 2Rb in An. gambiae and An. coluzzii allows karyotyping of both sexes and all developmental stages. These novel tools will accelerate deeper investigations into the role of this ecologically and epidemiologically important chromosomal inversion in vector biology.

Keyphrases

high throughput
contrast enhanced
magnetic resonance imaging
gene expression
copy number
computed tomography
zika virus
dna methylation
aedes aegypti
single cell
single molecule
gene therapy