Anticancer Screening of Ru(II) Photoredox Catalysts at Single Cancer Cell Level.
Zhongxian FanJiaen XieRajesh KushwahaShaoxia LiangWenqing LiArif Ali MandalLi WeiSamya BanerjeeHuaiyi HuangPublished in: Chemistry, an Asian journal (2023)
The rapid efflux of Pt-based chemotherapeutics by cancer cells is one of the major causes of drug resistance in clinically available drugs. Therefore, both the high cellular uptake as well as adequate retention efficiency of an anticancer agent are important factors to overcome drug resistance. Unfortunately, rapid and efficient quantification of metallic drug concentration in individual cancer cells still remains a tricky problem. Herein, with the help of newly developed single cell inductively coupled plasma mass spectrometry (SC-ICP-MS), we have found that the well-known Ru(II)-based complex, Ru3, displayed remarkable intracellular uptake and retention efficiency in every single cancer cell with high photocatalytic therapeutic activity to overcome cisplatin resistance. Moreover, Ru3 has shown sensational photocatalytic anticancer properties with excellent in-vitro and in-vivo biocompatibility under light exposure.
Keyphrases
- mass spectrometry
- visible light
- energy transfer
- single cell
- highly efficient
- capillary electrophoresis
- high performance liquid chromatography
- liquid chromatography
- reduced graphene oxide
- loop mediated isothermal amplification
- rna seq
- ms ms
- high resolution
- emergency department
- reactive oxygen species
- drug induced
- adverse drug
- tandem mass spectrometry
- sensitive detection