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Slow water dynamics in dehydrated human Jurkat T cells evaluated by dielectric spectroscopy with the Bruggeman-Hanai equation.

Hiroaki MatsuuraKiyoshi TakanoRyo Shirakashi
Published in: RSC advances (2023)
The picosecond orientational dynamics of intracellular water was measured by dielectric spectroscopy, with the aim of revealing the effects of cryoprotective agents (CPAs) on biological cells. As a first step, Jurkat cells (human T lymphocyte cells) suspended in aqueous sucrose solutions of different concentrations ranging from 0.3 M (isotonic) to 0.9 M (hypertonic) were examined at 25 °C with a frequency range up to 43.5 GHz. The Bruggeman-Hanai equation was employed to obtain a cellular dielectric spectrum without extracellular contributions from the measured complex permittivity of the cell suspensions. By analyzing the γ process around 10 10 Hz based on the Debye relaxation function, two types of water (bulk-like water and hydration water with slower molecular dynamics) were observed. An increase in the fraction of intracellular slower water was observed in the dehydrated cells which had a highly concentrated environment of biomolecules.
Keyphrases
  • induced apoptosis
  • cell cycle arrest
  • molecular dynamics
  • endoplasmic reticulum stress
  • signaling pathway
  • single molecule
  • cell death
  • oxidative stress
  • mesenchymal stem cells
  • ionic liquid
  • density functional theory